International Journal of Biosciences || IJB - INNSPUB

IJB_INNSPUB shared original research work that published by the journal named International Journal of Biosciences, an affiliated journal of International Network for Natural Sciences | INNSPUB

Some quality parameters of kordofan groundnuts (Arachis hypogaea L.) oil in relation to sudanese standards | IJB_INNSPUB

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By: A. M. Babeker, A. A. Abdalla, A. I. Ahmed, A. R. Ahmed

Key Words: Groundnuts oil, Good manufacturing practices (GMP), Physicochemical proprieties, Greater kordofan, Sudan

The present work was conducted to evaluate the physicochemical properties of the native groundnut oil processed in Kordofan region in order to investigate the effect of environmental processing and handling on quality (Good Manufacturing Practices -GMP-) with respect to SSMO, 1995 and Codex Alimentarius, 2005. Ten samples of groundnut oil were collected from the four major oil producing localities of the Greater Kordofan region i.e. Sheikan (Five expellers), Elnuhud (Three expellers), Elrahad (One), and Um Rowaba (One expeller). The oil samples (two samples from each expeller) were collected and kept in dry bottles sealed tidily and labeled, then transferred to the laboratory to assess the quality parameters, which include color degree, viscosity, refractive Index, density, impurities, moisture content, acid value, FFA%, peroxide, anisidine and Totox values.

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In vitro evaluation of synergistic antimicrobial activity of Trachyspermum ammi and Syzygium aromaticum | IJB_INNSPUB

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By: Kausar Malik, Hamid Bashir, Muhammad Bilal, Husnain Qamar, Zaid Mehmood

Key Words: Syzygium aromaticum (Cloves), Trachyspermum ammi (ajwain), Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli.

A remarkable Increase in antibiotic toxicity and multidrug resistance among several group of microorganism are the major challenge worldwide. These new emerging trends led for development of different strategies. Cloves (Syzygium aromaticum) and ajwain (Trachyspermum ammi) are plant species widely used in traditional medicine to treat various infectious diseases.

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In vivo anticancer activities of Co(II)-Benzoin Thiosemicarbazone Complex [Co(BTSC)2] | IJB_INNSPUB

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By: Hossain Mohammad Zakir, Md. Khairul Islam, Md. Rakib Hasan, Shaikh M Mohsin Ali, Mele Jesmin

Key Words: Antineoplastic activity, EAC cells, Cobalt benzoin thiosemicarbazone complex, Hematological prameters, Morphological change.

Cancer, a diverse group of diseases characterized by the proliferation and spread of abnormal cells, is a major worldwide problem. In order to find out new anticancer drug Co(II) complex with benzoin thiosemicarbazone was synthesized and characterized. Ehrlich Ascites Carcinoma (EAC) cells were used to evaluate the anticancer activities of benzoin thiosemicarbazone complex with Co(II) in swiss albino mice. The anticancer activities were studied by monitoring tumor cell growth inhibition, tumor weight measurement, survival time of tumor bearing swiss albino mice.

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Correlation analysis of morphological and fiber quality traits in upland Cotton (Gossypium hirsutum L.) | IJB_INNSPUB

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By: Sajid Fiaz

Key Words: Cotton, Fiber quality, Linkage analysis, Morphological

Fiber quality is the main consideration for ginners and most complex trait for breeder point of view as it depends on interaction of genetic makeup of plant and environment. To meet this objective, two parents B557, FH153 and their F2 population were evaluated for the existence of genetic variability for the characters under study during 2014-2015, at University of Agriculture, Faisalabad. The experiment was carried out in a randomized complete block (RCB) design with three replications. Data were recorded for Plant height (cm), Internodal distance (cm), Number of monopodia, Number of sympodial, Leaf hairiness, Pedicel length (cm), Boll size, Number of bolls per plant, Boll weight (g), Ginning out turn (%), Fiber fineness (µg/inch), Fiber strength (g/tex), Staple length (mm), Seed cotton yield per plant (g).

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Sequential aerobic-anaerobic treatment of municipal wastewater: effect of different parameters | IJB_INNSPUB

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By: Syeda Amber Hameed, Safia Ahmed, Naeem Ali

Sequential application of aerobic and anaerobic system for domestic wastewater treatment experiences certain limitations under low temperature regime and further requires optimization of exogenous inoculum size and treatment time. In order to address the aforesaid issues, a sequential aerobic-anaerobic digester was locally designed and operated for 8-14 days for wastewater treatment. Overall, the treatment efficiency varied from 92-100 % in terms of biochemical oxygen demand (BOD), chemical oxygen demand (COD), turbidity and NO2-N at 25ºC (non-significantly differed at 45ºC). Increase in aerobic retention time from 1-3 days helped improvement in treatment efficiency by 5-20 % in most of the parameters. However, a slight increase in COD (94 – 97%) and BOD (95- 97%) removal was observed when temperature raised from 25 to 45ºC. Whereas, almost 60% decrease in treatment efficiency was observed in term of BOD and COD removal when temperature decreased from 45-5ºC.

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Evaluation of alpha-fetoprotein in patient with chronic hepatitis C infection by using ELFA technology | IJB_INNSPUB

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By: Bakht Biland, Muhammad Waqar, Muhammad Wasim, Zobaria Rehman, Agha Asad Noor, Shahzeray Mukhtar, Qazi Shah Rukh, Noor Ul Akbar

Key Words: AFP, HCC, KPK, HCV, ELFA

Hepatitis known as inflammation of liver is caused by some known viruses, alcohol, drugs and some other infection. Most common hepatitis infections in humans are Hepatitis A, B and C. Chronic HCV infection may lead to liver cirrhosis and Hepatocellular carcinoma (HCC). Alfa feto protein’s concentration in patient’s serum is useful utility to diagnose HCC. The current study was conducted at Genome Research Centre Lahore for the measurement of AFP concentration in HCV positive patients of Punjab and Khyber Paktun Khwa (KPK) Province of Pakistan. Total of 172 patients, atleast 60 from each province were screened for Alfa feto protein concentration in their blood by using modern ELFA technology that is most rapid, specific and sensitive method.

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Antibacterial and antifungal activity of Cannabis sativa L., Mentha longifolia L. and Ricinus communis L. | IJB_INNSPUB

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By: Imran Khan, Muhammad Sabir

Bacterial and Fungal diseases still possesses a serious threat to public health. In this study the antibacterial and antifungal potential of crude methanolic extracts of Cannabis sativa (C. sativa), Mentha longifolia (M. longifolia) and Ricinus communis (R. communis) was investigated. The antibacterial activity was determined using agar well diffusion method against two gram positive i.e. Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis) and gram negative bacterial strains i.e. Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa). The antifungal activity was invistigated by using agar tube dilution method aginst two fungal strains i.e. Aspergillus niger (A. niger) and Aspergillus flavous (A. flavous). In antibacterial activity, all the plants extracts showed higher bacterial inhibition against gram negative strains compared to gram positive strains.

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