Performance of GSR genotype as influenced by different crop establishment methods | IJB_INNSPUB

By: H. Ferdous, M. R. Karim, P. S. Saha, M. J. Rahman, M. A. Kabir, M. S. Islam

Different crop establishment methods are used in Asian rice cultivation system. Though transplanting is the most popular method, farmers also tend to direct seeding to reduce production cost. A field trial was conducted to evaluate the performance of newly selected Green Super Rice (GSR) genotype under dry direct line seeding, wet direct line seeding and conventional tillage puddle transplanting methods at BRAC Agricultural Research and Development Centre (BARDC), Gazipur during Boro 2012-13 and Aman 2013. . Performance of HHZ-15-DT7-SAL4-SAL1 was evaluated in both the seasons. Two BRRI released varieties BRRI dhan28 and BRRI dhan39 were used as check in Boro and Aman, respectively.

Effect of explants and growth regulators on the expression of Callogenesis, somatic embryogenesis and plantlets formation in Sugarcane (Saccharum officinarum L.) | IJB_INNSPUB

By: Muhammad Iqbal, Aamir Ali, Naima Huma Naveed, Umair A. Khan, Muhammad Nasim Ashraf Faz, Muhammad Imran, Danish Ashfaq, Mubashir Hussain

The present research was done to standardize a protocol for hormone treatment and to determine the best type of explants for the induction of callus, somatic embryogenesis and regeneration response in sugarcane. The potential for callogenesis and somatic embryogenesis of different explants of sugarcane was explored. The influence of different concentrations and combinations of various phytohormones along with varying environmental conditions like temperature and light on in vitro callus induction and somatic embryo formation was also studied. Among three types of explants used in present study (leaf, shoot apical meristem and pith), the maximum response of callus induction and somatic embryogenesis was obtained from leaf explants.

Bio-efficacy of Pseudomonas fluorescens isolated from chickpea fields as plant growth promoting rhizobacteria | IJB_INNSPUB

By: Shazia Shahzaman, Muhammad Inam-Ul-Haq, Shagufta Bibi, Muhammad Sufyan, Adeela Altaf, Umair Mehmood, Raees Ahmed

Chickpea is an economically important food crop, which is subjected to infection by a host of fungal, viral and bacterial pathogens. Thirty isolates of Pseudomonas fluorescens were isolated from the rhizosphere of Chickpea fields. These were tested against F. oxysporum in dual culture method. Among these, four (Pf 1, Pf 3, Pf 5 and Pf 8) isolates were showed bright fluorescence under UV light were further tested. All the cultural and biochemical studies confirmed them to be P. fluorescens.

Protocol on direct organogenesis in Chrysanthemum morifolium Ramat | IJB_INNSPUB

By: Rawnak Jahan Swarna, Dilruba Yeasmin, Md. Mostafizur Rahman, Md. Firoz Alam

An efficient direct organogenesis protocol was developed for Chrysanthemum morifolium Ramat., an ornamental herb.The purpose of this study was todetermine the effect of different growth regulators on shoot formation and multiplication over the cultural periodand mass propagation in large quantities in a short timeusing tissue culture techniques.Nodal segments, after being sterilized with 0.1% HgCl2(mercuric chloride) for eight minutes, were inoculated in MS media (Murashige and Skoog, 1962)with different concentrations of BAP(6-benzyl amino purine) singly and combinations with KIN (6-furfural amino purine), Casein hydrolysate (CH), NAA (α-napthalene acetic acid).

Evaluation of the biochemical and phytochemical components of green seaweed Enteromorpha intestinalis (Linnaeus) in Initao, Misamis oriental, Mindanao, Philippines | IJB_INNSPUB

By: Hansen Rose S. Escobido, Maria Luisa S. Orbita, Ronaldo R. Orbita

Key Words: Iligan Bay, Saponin, Steroid, Alkaloid

The green seaweed Enteromorpha intestinalis that occurred along the coast of Iligan Bay was analyzed for biochemical and phytochemical components. Powdered samples of E. intestinalis were analyzed for carbohydrate, protein, fat, ash and moisture content. Likewise, the presence of reducing sugar, tannins, phenols, saponins, anthraquinones, flavonoids, steroids, terpenoids and alkaloids were determined using the standard methods.

Effect of Mg,Cu,Cd and Mg/Cd, Cu/Cd on stress biomarkers in durum wheat. | IJB_INNSPUB

By: N. N. Azizi, M. R. Djebar, H. Sbartai

This work aims to evaluate the effects of few trace metals (Cd,Cu) and a major element (Mg) and their interactions (Cd/Cu and Cd/Mg) on the growth rate of durum wheat roots (Var. Simeto) and stress biomarkers: glutathione and catalase activity (CAT) in the roots and leaves of durum wheat. A treatment with concentrations (5,20,50μM) of Cu and Mg combined or not to (100 µM) of Cd is applied. The results show an increase growth rate for all Mg concentrations whereas copper has stimulating at low concentrations and inhibiting at high concentrations. As for combinations Mg/Cd and Cu/Cd, inhibit root growth except at [100 µM] of Cd stimulating it during the first 96 hours. However GSH is stimulated at low [Mg/Cd] and increasing concentrations of Cu except the lowest dose where no significant increase is observed.

Assessment of genetic diversity of wheat (Triticum aestivum L.) using agro-morphological characters and microsatellite markers | IJB_INNSPUB

By: Karima Kara, Najla Mezghani, Olfa Saddoud Debbabi, Maher Madini, Malika Rached-Kanouni, M’barek Ben Naceur

Genetic diversity of ten bread wheat (Triticum aestivum L.) varieties was evaluated at the DNA level using 16 wheat microsatellites molecular markers (Simple Sequence Repeat-SSR) and 6 agro-morphological characters. SSR bands were scored across all genotypes, for presence (1) or absence (0) and transformed into 0/1 binary matrix. A pair-wise similarity matrix was generated with the software NTSYS. The Polymorphism Information Content (PIC) ranged from 0.13 to 0.70 respectively for the primer WMC 24 and WMC 50 with an average of 0.48 and 0.49 per primer pair. The similarity coefficient between cultivars ranged from 0.33 and 0.90 with an average of 0.63. Most of the genotypes showed a high degree of genetic similarity.