Estimation of Genetic Diversity among Oat Genotypes through Agro-morphological Traits | IJB_INNSPUB

By: Muhammad Tehseen Zaheer Tanoli, Sardar Ali, Nadar Khan, Malik Ashiq Rabbani, Sher Aslam Khan, Shah Masaud Khan, Khalid Naveed, Muhammad Arif

Key Words: Correlation, Oat, Genetic diversity, Principal component analysis, Cluster analysis

During the present study diverse germplasm comprising 124 oat accessions including three checks was evaluated for various quantitative agro-morphological traits in the research field of PGRI, NARC, Islamabad during the year 2013-14. The principal objective of the research was to study the magnitude of genetic diversity and relationships between different genotypes and to identify promising genotypes of oat for the traits of economical importance. The seed material was collected from diverse ecologies of Pakistan and USA. Most of the traits showed considerable variations.

Effect of reduced impact logging to species composition and forest structure in tropical rain forest, North Borneo | IJB_INNSPUB

By: Muhdi, Elias, Daniel Murdiyarso, Juang Rata Matangaran

Key Words: Reduced impact logging, Conventional logging, Species composition, Tropical rain forest

Forest structure and species composition is the use of sustainable forest management, such as logging methods and underplanting. Diversity of species in tropical forest is very large and complex, its existence influence each other and interact with a genetic characters and the ecosystem. Measuring the impact of logging techniques of reduced impact logging (RIL) and conventional logging (CL) performed on the logging block of the current year. Plots consisted of CL and RIL block that the size of plot 15 ha, respectivly.

Molecular characterization and 16S rRNA sequence analysis of probiotic lactobacillus acidophilus isolated from indigenous Dahi (Yoghurt) | IJB__INNSPUB

By: Wajiha Farid, Tariq Masud, Asma Sohail, Saqlain Naqvi, Mudassir Ali Qazalbash

Key Words: Probiotic, Lacidophilus, Acid and bile tolerance, Antimicrobial activity, 16S rRNA PCR assay, Sequence analysis.

Indigenous yoghurt is being the most popular fermented milk product in Pakistan, produced by heterogenous lactic acid bacteria (LAB). Nowadays LAB has drawn particular interest in food and nutrition science because of functional and probiotic attributes. Among various genera of LAB, Lactobacillus acidophilus is considered to be a potential candidate. Present study was designed by keeping in view the probiotic attributes of L. acidophilus. Isolation was conducted on MRS media supplemented with 0.7% bile salts to screen out bile tolerant isolates. Out of sixty lactobacilli, eighteen were identified as L. acidophilus by performing gram staining, catalase, carbohydrate fermentation test and growth at different temperatures.

Horizontal and vertical emissions of methane from a drained tropical peat soil cultivated with Pineapple (Ananas comosus (L.) Merr.) | IJBINNSPUB

By: A. V. Jeffary, O. H. Ahmed, R. K. J. Heng, L. N. L. K. Choo

Key Words: Emissions, Horizontally, Methane fluxes, Tropical peatlands, Vertically

Drained tropical peat soils especially for agricultural purposes could lead to methane (CH4) emission into the atmosphere. Methane emission from peat soils to the atmosphere depends on rates of methane production, consumption and ability of the soil and plants to transport the gas to soil’s surface and also within soil particles. The objective of this study was to determine CH4 fluxes horizontally and vertically from the floor and wall of the pit of a tropical peat soils cultivated with Ananas comosus (L.) Merr. and to determine the relationship between CH4 transportation and CH4 emission from a drained tropical peat soils. Gas samplings were conducted in the dry and wet seasons.

Growth response of warm season grass (Cynodon dactylon) to different salinity levels | IJB_INNSPUB

By: Zainab Akhtar, Usman Shoukat Qureshi, Saman Chughtai, Sawal John, Ali Raza Mir, Azqa Azhar Qureshi

Key Words: Turfgrass quality, Salinity, Tolerance

Turfgrasses are essential natural element of any landscape, which play a dynamic role in redecoration of gardens, parks, golf course and play grounds. Cynodon dactylon L., cv. Tiffany is a warm season turfgrass having vibrant value and reputation because of its stunning appearance, hardy nature, soft carpet sensation, drought, salinity resistance quality, heavy traffic bearing and browning . In which Salinity is one of the major environmental factor which limits turf growth and quality because it reduces water availability for plant use. High salt levels hinder water absorption, inducing physiological drought in the plant. Scarcity of fresh water along with soil salinization has resulted in an increased need for screening of salt tolerant turfgrasses which have the ability to withstand against different salinity levels.

The effect of birth type and sex of kids on milk lactose levels | IJB_INNSPUB

By: Yeliz Çakir Sahilli, Murat Çimen

Key Words: Goat, Milk, Lactose, Birth type, Sex

This research was conducted in order to learn the effects of birth type (single or twins) in goats and sex of kids upon milk lactose levels. Therefore, 14 native goats (kıl) based on birth type and another 14 native goats (kıl) based on sex of kids were divided into two groups. Milk sample for once was obtained from the kids in the 3rd and 4th weeks of lactation. Lactose levels were specified as the mean of two weeks.

Cell free DNA – A novel biomarker in the field of oncology: A comparative account in the cancer patients and healthy residents of Karachi city

By: Mohammad Attaullah, Masarrat J. Yousuf, Saifullah Afridi, Islam Dad, Muhammad Amin, Syed Ishtiaq Anjum, Rooh Ullah

Key Words: Cell free DNA, Serum, Cancer, Biomarker, Nano drop analyzer, Circulating DNA

The present study was carried out to ascertain the levels of cell free DNA (cf-DNA) in the serum samples of diagnosed cancer patients having various malignancies and healthy residents of Karachi City. Collection of blood samples was carried out with informed consent from diagnosed cancer patients and healthy human subjects at various hospitals of Karachi City. Serum was isolated and analyzed for cf-DNA with genomic DNA isolation accompanied with Isoamyl-Phenol-Chloroform purification.