Diagnosis of the floristic diversity of Ain Skhouna (Steppe West-Algerian) | IJB_INNSPUB

By: Anteur Djamel, Mederbal Khelladi, Z. Belhacini Fatima, Baghdadi Djilali, Gourari Bariza, Marouf Baghdad

Key Words: Diversity, Flora, Degradation, Ain Skhouna, West-Algerian

This work allowed making an assessment of the floristic diversity of Ain Skhouna, in Western Algeria, based on the phytoecological aspect, biological and biogeographically of this heritage. Given the nature of the problem to be addressed, we used the Zuricho Montpelieraine developed by Braun-Blanquet method, this method is to harvest all plant species encountered, and list species on a surface plot (IEA minimum), the floristic surveys according to the method of Braun-Blanquet.

Comparative microarray data analysis of Arabidopsis genome during interaction with a mutualistic and a pathogenic bacteria | IJB_INNSPUB

By: Nazeer Ahmed, Shahjahan Shabbir Ahmed, Fazalur Rehman, Muhammad Naeem Shahwani, Muhammad Saeed, Imran Ali Sani, Muhammad Naseem

Key Words: Microarray, Mutualism, Pathogenesis, Expression profiling.

Plants are in continuous interaction with both mutualistic and pathogenic microorganisms, particularly in the rhizosphere. Arabidopsis has served as an excellent model plant in a variety of experiments to determine the details and nature of such relationships. Studies involving transcriptome profiling of Arabidopsis thaliana in response to pathogenic and beneficial bacterial infection are available, each focusing primarily on defense related and plant growth promotion related genetic component respectively. In an attempt to decipher the difference in responses of plants to these two types of bacteria, we resorted to genome wide comparative analysis both when it was exposed to a foe and a friend.

Influence of nitrogen level and planting time on growth of Aloe vera plant | IJB_INNSPUB

By: Khaliq Ur Rehman, Riffat Un Nisa, Burhan Ali, Jawad Ali, Anwar Rahid, Abid Khan, Mohammad Ilyas

Key Words: Alovera (Aloe barbadensis), Nitrogen levels, Planting, Number of leaves, Plant height, Leaf thickness

An experiment was carried out at newly developmental farm (horticulture section) situated at University of Agriculture, Peshawar during the year 2012 to see whether the influence of nitrogen level and planting time on growth of Aloe vera plant was or not. The experiment was carried out using Randomized Complete Block Design with split plot arrangement. There were four levels of nitrogen (0, 50, 100 and 150 Kg ha-1) and four planting times (20th March, 04th April, 20th April and 04th May, 2012). The Urea (46% nitrogen) was applied as a source of nitrogen. Nitrogen levels significantly affected all of the parameters except survival percentage while planting time affected all parameters significantly.

Incidence of multidrug resistant coagulase negative Staphylococci in clinical samples of Tertiary care hospitals of Khairpur and Sukkur cities of Pakistan | IJB_INNSPUB

By: Shahida Mangi, Anwar Hussain Phulpoto, Muneer Ahmed Qazi, Nisar Ahmed Kanhar

Key Words: Coagulase-negative staphylococci, Multidrug resistance, Antimicrobial sensitivity profiling, Clinical samples, Tertiary care hospitals

Coagulase-negative staphylococci (CoNS) adopting multidrug resistance have been increasingly becoming a threat to the patients in tertiary care hospitals worldwide. The aim of present study was to find out the incidence of multidrug-resistant CoNS in various samples of clinical origin. A total of 280 clinical samples of different origin were collected from tertiary care hospitals of Khairpur and Sukkur cities of Pakistan. Coagulase-negative staphylococci (CoNS) were isolated and identified using routine microbiological techniques and molecular characterization using 16S rRNA sequence-based homology.

Effect of various drying techniques on certain characteristics of apricot fruit | IJB_INNSPUB

By: Zulfiqar Ali, Abid Hussain, Maqsood Husain, Syeda Mashal, Saqib Hussain

Key Words: Apricot, Food safety, Ascorbic acid, Ash content, Total sugars

Man is innovating new tools and techniques to fulfill nutritional and food safety concerns for the growing population. Six drying methods were applied on apricot fruit to analyze their effect on certain characteristics of dried samples. The findings for open shade drying, open sun, sulfuring, solar house, portable solar house and cabinet drying for moisture content were 13.3±0.06, 11.3±0.12, 18.90±0.10, 7.7±0.06, 13.0±0.06, 11.0±0.10g/100g, pHfoundas4.00±0.00, 3.00±0.00, 3.00±0.00, 3.00±0.00, 4.00±0.00, 3.00±0.00),

Nutrient digestibility and ruminal fermentation of an unconventional feed estimated by the in vivo techniques | INNSPUB_NET

By: Ping Sheng, Zhihong Zhang, Jiangli Huang, Dongsheng Wang, Yizun Yu, Guohua Zhang, Shaoxun Tang, Jiannan Ding

Chemical composition, feed intake and apparent digestibility of medicinal plant residue (MPR) used for ruminants were evaluated.MPR had higher crude protein (CP), crude fat (EE) and lower crude fiber (CF), neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents than those of the roughage feed-peanut vines. Compared to the concentrated feeds, MPR showed a lower CF than that of other feeds except the maize, and NDF and ADF contents were also lower than those of other feeds except the soybean meal.

Evaluation of cells integrity using different fixation time by scanning electron microscopy | IJB_INNSPUB

By: H. L. Hing

Key Words: Scanning electron microscopy, HT-29, CCD-18Co, Lactobacillus.

The study aims to evaluate the integrity and structural changes of two intestinal cells adhered with Lactobacillus sp. in different fixation time. HT-29 and CCD-18Co intestinal cells with Lactobacillus sp. were fixed with 2.5% glutaraldehyde in 0.1 M phosphate buffer for two, four, six and 12 hours at room temperature. Our study revealed that the different fixation time affects and change the integrity, viability and durability of cells.